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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence information is not really but offered.
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Blocking peptides are peptides that bind precisely into the concentrate on antibody and block antibody binding. These peptide ordinarily incorporates the epitope acknowledged from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to make certain the absolute best outcomes are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be sure the best possible effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining within the blocked antibody versus the antibody on your own, one can see which staining is precise.
Concentrate on Information Cyclin E belongs on the very conserved cyclin family members, whose associates are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which add on the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates for the G1-S section boundary which is degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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